γ-Glutamylamine derivatives in isolated rat hepatocyte proteins
نویسندگان
چکیده
منابع مشابه
Identification of rat hepatocyte plasma membrane proteins using monoclonal antibodies
We have localized and identified five rat hepatocyte plasma membrane proteins using hybridoma technology in combination with morphological and biochemical methods. Three different membrane preparations were used as immunogens: isolated hepatocytes, a preparation of plasma membrane sheets that contained all three recognizable surface domains of the intact hepatocyte (sinusoidal, lateral, and bil...
متن کاملIdentification of Rat Membrane Proteins Hepatocyte Plasma Using Monoclonal Antibodies
We have localized and identified five rat hepatocyte plasma membrane proteins using hybridoma technology in combination with morphological and biochemical methods. Three different membrane preparations were used as immunogens: isolated hepatocytes, a preparation of plasma membrane sheets that contained all three recognizable surface domains of the intact hepatocyte (sinusoidal, lateral, and bil...
متن کاملHepatic pyruvate kinase. Quantitative measurements of phosphorylation in vitro and in the isolated rat hepatocyte.
The phosphorylation of rat liver pyruvate kinase was studied in isolated rat hepatocytes and with the crystalline enzyme. Hepatocytes were incubated with ["PI orthophosphate and isotopically labeled pyruvate kinase was quantitatively isolated using a resin-coupled antibody. The molar extent of phosphorylation of the enzyme was calculated from the amount of radioactivity in the enzyme and the sp...
متن کاملInteraction of Probenecid with Methotrexate Transport and Release in the Isolated Rat Hepatocyte in Suspension1
Probenecid has been shown to delay the plasma clearance of methotrexate in the rat and to reduce both hepatic and renal excretion of methotrexate in this animal model. In order to probe the mechanism by which probenecid alters hepatic excretion of the antifolate, studies assessed the effects of probenecid on transport, accumulation, distribution, and release of methotrex ate in the rat hepatocy...
متن کاملInteraction of probenecid with methotrexate transport and release in the isolated rat hepatocyte in suspension.
Probenecid has been shown to delay the plasma clearance of methotrexate in the rat and to reduce both hepatic and renal excretion of methotrexate in this animal model. In order to probe the mechanism by which probenecid alters hepatic excretion of the antifolate, studies assessed the effects of probenecid on transport, accumulation, distribution, and release of methotrexate in the rat hepatocyt...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1988
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2490813